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. 2011 Jun;85(12):5919–5928. doi: 10.1128/JVI.00116-11

Fig. 4.

Fig. 4.

Imaging of capsids and gB in axons of SCG rat neurons present in the connecting channels between microfluidic chambers. Rat SCG neurons were plated in the somal chambers of microfluidic devices (20,000 neurons per chamber) for 14 days. The cells were infected with YK614 using 3 PFU/cell and were then imaged by live-cell deconvolution microscopy after 23 h. (A) VP26-Venus-labeled capsid (Separate particle) moving in the anterograde direction, from left to right. (B) VP26-Venus labeled capsid with colocalized mRFP-gB fluorescence (Married particle) moving in the anterograde direction. The small displacement of green and red signals relates to the need to switch filters in order to detect different fluorescent wavelengths. (C) Puncta containing mRFP-gB moving in the anterograde direction. Gaps were introduced, e.g., in panel A between frames 7 and 26, in order to show longer distances of travel. Frames of 0.5 s are numbered along the right side. Bars, 5 μm.