Table 1.
Colocalization of fluorescence in YK614 virus particles from cell culture supernatants of Vero cellsa
| Fluorescence | Colocalizationb |
|---|---|
| mRFP-gB | 221/276 (80) |
| R#68 (rabbit polyclonal anti-gB) | 152/210 (72) |
| DL6 (mouse anti-gD MAb) | 165/211 (78) |
| 3B6 and H1.4 (anti-VP5 MAb) | 222/263 (84) |
| NC-1 (rabbit polyclonal anti-VP5) | 197/225 (88) |
| NC-7 (rabbit polyclonal anti-VP26) | 178/198 (90) |
a
YK614 (expressing both mRFP-gB and VP26-Venus) particles from cell culture supernatants were allowed to adhere to polylysine- and laminin-coated glass coverslips; then they were fixed with 4% PFA and permeabilized with 0.2% Triton X-100 before being imaged directly (VP26-Venus versus mRFP-gB) or stained with various antibodies.
b
Expressed as the number of capsids detected by VP26-Venus fluorescence that exhibited mRFP-gB fluorescence or stained with the anti-gB, anti-gD, anti-VP5, or anti-VP26 antibodies/total number of capsids detected by VP26-Venus fluorescence (percentage).