Table 2.

Summary of the anterograde and retrograde transport of HSV capsids in SCG rat neurons in microfluidic chambers

Expt no.	Expt descriptiona	No. of capsidsb
		Anterogradec		Retrograde		Static
		Married	Separate	Married	Separate	Married	Separate
1	40,000 neurons, large bundles of axons, 7 days in culture before infection using 3 PFU/cell, imaging begun at 20 h p.i.	20	46	3	55	4	38
2	15,000 neurons, fewer axons in channels, 14 days in culture before infection using 3 PFU/cell, imaging begun at 19 h p.i.	4	31	0	0	3	1
3	20,000 neurons, fewer axons in channels, 14 days in culture before infection using 12 PFU/cell, imaging begun at 22 h p.i.	35	61	1	6	0	6
4	20,000 neurons, moderate bundling of axons in channels, 14 days in culture before infection using 3 PFU/cell, imaging begun at 23 h p.i.	16	14	1	2	41	37

^aYK614 was used for infection in all experiments. p.i., postinfection.

^bCapsids detected by VP26-Venus fluorescence that exhibited mRFP-gB fluorescence (Married) or that showed no detectable mRFP-gB fluorescence (Separate) were enumerated. Anterograde, capsids that moved exclusively in the anterograde direction; retrograde, capsids that moved in the retrograde direction during the majority of their transport; static, capsids that remained static for all of the observation period.

^cA total of 75 capsids (33%) moving in the anterograde direction were Married, and 152 (67%) were separate, in the four experiments taken together.