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. 2011 Jun;85(12):5940–5948. doi: 10.1128/JVI.00193-11

Fig. 4.

Fig. 4.

C1q is required for NHS-mediated enhancement of neutralization of NiVpp by anti-NiV antibodies. (A) NiVpp were applied to carbon-coated grids and treated with combinations of purified C1q or polyclonal antibody specific for NiV F and G proteins. Samples were subsequently treated with gold-conjugated anti-C1q (6-nm beads) or anti-IgG (12-nm beads) before they were examined by electron microscopy. (B) NHS-mediated enhancement of neutralization requires C1q. NiVpp were incubated for 1 h with the indicated combinations of polyclonal antibody against F and G, C1q-depleted serum, and physiological levels of C1q. The remaining infectivity was determined by infecting Vero cells and assaying luciferase activity. Error bars show standard deviations. #, P < 0.001.