Fig. 2.
Western blot analysis of SHWT and SHSTOP expression. SH-HA expression (anti-HA antibody [1/1,000]; Roche, Indianapolis, IN) was observed in cells transfected with pSHWT-HA (lane 3) but not in cells transfected with pSHstop-HA (lane 1). Vero extracts isolated from cells transfected with the pCAGGS vector served as the assay control (lane 2). Expression of a cellular marker, glyceraldehyde-3-phosphate dehydrogenase (GAPDH; anti-GAPDH antibody [1/5,000]; Abcam, Cambridge, MA), was detectable in all samples.