Skip to main content
. 2011 Jun;85(12):6015–6023. doi: 10.1128/JVI.01537-10

Fig. 7.

Fig. 7.

siRNA knockdown of Bcl-xL in NDV-infected cells reduces oncolytic activity. (A) Western blotting of Bcl-xL knockdown using different amounts of siRNA. (B) A549–Bcl-xL cells were transfected with 5 nM Bcl-xL siRNA or scrambled siRNA and then infected with NDV (LS)-GFP 24 h later. Images were acquired by fluorescence microscopy to assess syncytium formation. (C) Bcl-xL siRNA 575-transfected cells were infected with NDV at an MOI of 0.1, and titers were assessed at 24, 48, and 72 h. *, P < 0.01. (D) MDA-MB-231 and MDA-MB-468 cells were transfected with 5 nM Bcl-xL siRNA and infected with NDV (LS)-GFP at an MOI of 0.1. Titers were assessed at the indicated time points. MDA-MB-231: *, P < 0.004; **, P < 0.002; ***, P < 7 × 10−6. MDA-MB-468: *, P < 0.02; **, P < 0.001; ***, P < 0.0009.