Fig. 1.

M51R VSV requires a Fas-mediated pathway to induce apoptosis in GBM cells. (A) U87 cells that were not transfected, empty vector clone 8 (EV-8), and dominant negative Fas clone 7 (dnFas-7) cells were infected with M51R VSV for various periods of time. Cell extracts were prepared, and caspase-3-like activity was assessed. (B) U87, EV-8, and dnFas-7 U87 cells were treated with ch.11 for 12 h and were analyzed as in panel A. (C) Prior to infection, U87 cells were treated for 1 h with DMSO, zVAD, or zIETD. Cells were infected with M51R VSV at various times, and cell extracts were prepared and analyzed as in panels A and B. Asterisks indicate statistical significance (P < 0.05) against EV or DMSO control cells at corresponding time points among three independent experiments. Error bars indicate standard errors of the means. RFU, relative fluorescence units.