Fig. 3.

Bid is required for apoptosis induced during M51R VSV infection in GBM cells. (A) U87 cells were pretreated with DMSO or zIETD prior to treatment with ch.11 (12 h) or at various times of infection with M51R VSV. Cell lysates were prepared, and Bid expression was determined by immunoblotting. (B) Densitometry analysis was preformed on immunoblots similar to those shown in panel A. (C) U87 cells were either mock transfected or transfected with Bid siRNA (siBid) or nontargeting siRNA (siNT), and efficiency of silencing was assessed by immunoblotting. (D) At 48 h posttransfection, cells transfected as in panel C were infected with M51R VSV, and caspase-3-like activity was assessed. (E) Cells transfected as in panel C were treated with ch.11 for 12 h, and caspase-3-like activity was measured. Asterisks indicate statistical significance (P < 0.05) against DMSO or nontargeting control cells at corresponding time points among three independent experiments. Error bars indicate standard errors of the means.