Fig. 3.

Induction of RELA allelic variants. RELA^−/− MEFs were transiently cotransfected with 1 μg warthog (clear box) or 1 μg domestic pig (filled box) RELA. Cells were treated with TNF-α (30 ng/ml), lipopolysaccharide (LPS; 10 μg/ml), phorbol-12-myristate 13-acetate (PMA; 20 mM), and hydrogen peroxide (10 μM) immediately after and for the duration of the transfection. Cells were harvested at 24 h. RELA-induced NF-κB–luciferase (1 μg) activity was normalized to cotransfected TK-Renilla luciferase (1 μg) activity and presented as the fold induction above nondrug-treated cells (relative value of 1 depicted by the line).