Fig. 4.

Comparison of individual allelic RELA variation on NF-κB activity. Comparison of NF-κB–luciferase activity in RELA^−/− MEFs after 24 h transient transfection with 1 μg warthog (WH) or 1 μg domestic pig (DP) RELA or 1 μg of RELA variants encoding the individual amino acid substitutions T448A, S485P, and S531P. Activity is presented as the fold difference between RELA-induced NF-κB–luciferase activity normalized to cotransfected TK-Renilla luciferase activity relative to that of the empty vector normalized to cotransfected TK-Renilla luciferase activity. Error bars = standard deviations from the means, *, P < 0.08 versus DP.