Fig. 7.

(A) Polymerase activity of WT and NS5 M10 using 5′ DV RNA as a template under conditions for nonspecific activity. (B) SDS-PAGE of purified M10.1 and M10.2 NS5 proteins (left). Polymerase activity of NS5 M10 individual mutants using 5′ DV RNA or poly(rC) as a template (right). Error bars indicate the standard deviations of results from three independent experiments. MW, molecular weights in thousands. (C) Elongation activity of NS5 with RNA primers of different lengths. NS5 was incubated with a 24-nucleotide RNA template and the indicated primers. RNA products of the reaction were run in a 20% polyacrylamide gel. A 24-nt RNA mobility marker (MK) is included. (D) Elongation activity of mutated NS5 recombinant proteins. The reaction was carried out using a 4-nucleotide RNA primer (5′-UUCU-3′) and the 24-nucleotide RNA template indicated on the top panel.