Fig. 2.

Plaque-purified rVSV expressing SRV-2 Gag and Env construct proteins. (A) rVSV glycoprotein exchange vaccine vectors expressing SRV-2 Gag protein were assayed using Western immunoblotting. Anti-FLAG antibodies were used to detect the expression. Appropriate cell and empty-virus-vector (EV) controls were maintained. (B) rVSV glycoprotein exchange vaccine vectors expressing SRV-2 EnvTrunc proteins and EnvGCYT proteins. The expressed proteins were detected using pooled sera from five known SRV-positive monkeys at the TNPRC. Goat anti-monkey HRP conjugate was used as the secondary antibody. Indiana (IND) and Chandipura (CHP) strains of VSV were used to clone SRV-2 Gag and Env fragments.