Fig. 4.

Transient expression of IE1-72 alone was enough to enhance HDR in HFFs. Cells were nucleofected with either pSG5 or pSG5IE1-72 and seeded onto glass coverslips. After 24 h, cells were mock or Ad infected. Coverslips were harvested 72 h later. Cells were stained by IF for the presence of IE1-72 using an anti-IE1 and anti-IE2 MAb Ch16.0 (IgG1; Rumbaugh-Goodwin Institute for Cancer Research, Inc.). (A) Representative IF staining for IE1-72⁺ and GFP⁺ cells. (B) Percentages of GFP⁺ and double-positive (GFP⁺ plus IE1-72⁺) cells. Bars represent the averages of four experiments. The differences in the percentages of GFP⁺ cells were considered statistically significant (*** = P < 0.05).