Fig. 3.

Disulfiram does not cause CD4⁺ T cell activation. (A) Effects of disulfiram and anti-CD3 plus anti-CD28 treatment on the sizes of resting CD4⁺ T cells. Cell size was measured by flow cytometry based on forward scatter at 24 h and 48 h. (B) Effects of disulfiram and DDTC on the expression of activation markers in primary resting CD4⁺ T cells, compared to the effect of anti-CD3 plus anti-CD28 costimulation. Data are means plus standard deviations of triplicate samples from 1 of 2 independent experiments. (C) Effects of disulfiram and DDTC on global DNA and RNA levels in primary resting CD4⁺ T cells, compared to the effect of anti-CD3 plus anti-CD28 costimulation. Hoechst 33342 and pyronin Y were used to stain DNA and RNA, respectively, and results were read using a flow cytometer.