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. 2011 Jun;85(12):5864–5879. doi: 10.1128/JVI.00155-11

Fig. 2.

Fig. 2.

Relative localization of markers for major secretory organelles, endocytic organelles, and ESCRT III-associated proteins in uninfected lung fibroblasts (HLF cells). (A) Markers of the ER (Bip), Golgi apparatus (GM130 and Mann II), TGN (p230), and early endosomes (EEA1). (B) Markers of early (EEA1) and recycling (TfR and Rab11) endosomes. Structures whose centers are dually positive for EEA1 and TfR and have TfR-rich domains on their surface are indicated with arrows. (C) Markers of ESCRT III-associated proteins (CHMP1A and Vps4). (D) Markers of late endocytic compartments (CD63 and LAMP1). Rabbit polyclonal antibody staining is shown in green, mouse MAb staining in red, and DAPI staining in blue. Grid spacings are indicated for each image. The corresponding videos, Video S2A and S2BCD, are available in the supplemental material.