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. 2011 Jun;85(12):5864–5879. doi: 10.1128/JVI.00155-11

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Copyright © 2011, American Society for Microbiology

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Fig. 3. — Relative localization of markers for major secretory organelles, endocytic organelles, and ESCRT III-associated proteins in HCMV-infected lung fibroblasts (HLF cells). (A) Markers of the ER (Bip), Golgi apparatus (GM130 and Mann II), TGN (p230), and early endosomes (EEA1). (B) Markers of early (EEA1) and recycling (TfR and Rab11) endosomes. (C) Markers of ESCRT III-associated proteins (CHMP1A and Vps4) relative to p230 and EEA1. (D) Markers of late endocytic compartments (CD63 and LAMP1) relative to EEA1. (E) Markers of ESCRT III-associated proteins (CHMP1A and Vps4) versus markers of late endocytic compartments (CD63 and LAMP1). Enlargements of examples of structures described in the text as having the appearance of a sausage wrapped in a bun are provided as insets in the Vps4/EEA1 (C) and Vps4/CD63 (E) images. The objects that were enlarged are indicated with arrows. Rabbit polyclonal antibody staining is shown in green, mouse MAb staining in red, and DAPI staining in blue. Grid spacings are indicated for each image. Corresponding videos, Videos S3A, S3BC, and S3DE, are available in the supplemental material.

Fig. 3. — Relative localization of markers for major secretory organelles, endocytic organelles, and ESCRT III-associated proteins in HCMV-infected lung fibroblasts (HLF cells). (A) Markers of the ER (Bip), Golgi apparatus (GM130 and Mann II), TGN (p230), and early endosomes (EEA1). (B) Markers of early (EEA1) and recycling (TfR and Rab11) endosomes. (C) Markers of ESCRT III-associated proteins (CHMP1A and Vps4) relative to p230 and EEA1. (D) Markers of late endocytic compartments (CD63 and LAMP1) relative to EEA1. (E) Markers of ESCRT III-associated proteins (CHMP1A and Vps4) versus markers of late endocytic compartments (CD63 and LAMP1). Enlargements of examples of structures described in the text as having the appearance of a sausage wrapped in a bun are provided as insets in the Vps4/EEA1 (C) and Vps4/CD63 (E) images. The objects that were enlarged are indicated with arrows. Rabbit polyclonal antibody staining is shown in green, mouse MAb staining in red, and DAPI staining in blue. Grid spacings are indicated for each image. Corresponding videos, Videos S3A, S3BC, and S3DE, are available in the supplemental material.