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The acetylation status of Psm3 modulates Wpl1-dependent cohesin removal from chromatin. All strains contain the cdc25-22 and mis4-367 mutations and were cultured and processed as in Fig. 1. (A to C) Chromatin-bound Rad21 was measured from nuclear spreads. The error bars represent SD from 3 to 5 independent experiments. (D to F) Kinetics of Rad21 dissociation from chromatin. Each data set (A to C) was normalized respective to its time zero value (arbitrarily set at 100).
