Fig. 5.

The PDZ domain of SNX27 is critical for interaction with NR2C. (A) Six SNX27 constructs (schematically depicted) were cloned in the pGBKT7 vector fused to GAL4BD and transformed into yeast AH109. The purified NR2C-CT in pGADT7 was retransformed into yeast Y187. (B) The indicated AH109 and Y187 yeast cells were mated and plated on leucine- and tryptophan-free SD plates (SD/−Leu/−Trp). To confirm interaction, mated yeast cells were grown on the highest-stringency medium lacking leucine, tryptophan, histidine, and adenine (SD/−Leu/−Trp/−His/−Ade).