Fig. 4.

Effect of Dpb3 on ssDNA dynamics during replicative senescence. Each of three independently generated tlc1Δ rad52Δ strains (with or without other mutations) was taken from germination plates, pooled at passage 1, and propagated in two groups: DPB⁺ (tlc1Δ rad52Δ strains) and dpb3Δ (tlc1Δ rad52Δ dpb3Δ strains). (A and C) ssDNA measured for DPB⁺ and dpb3Δ groups at Y′-1000 (A) and at Y′-5000 (C). Error bars represent the standard deviations (SD) for three measurements. (B) Same as in panel A, except that DNA was quantified at Y′-1000 and normalized to the level for DNA at the centromeric location PAC2. The same groups of strains was used for the measurements presented in panels A to C.