Table 1.
Sample purification step | Protein (mg/ml) | Sp act (AU/mg protein) against C. jejuni | Purity (%) |
---|---|---|---|
Culture supernatant | 1.3 | 435 | 0 |
Gel filtration on Superose 12 HR 10/30 | 0.02 | 80,000 | 13.3 |
Binding to SP-Sepharose FF | 0.015 | 107,000 | 76.4 |
Hydrophobic-interaction chromatography on Phenyl Sepharose 6 Fast Flow | 0.007 | 229,000 | 95.7 |