Table 1.
Targets and sequences of the primer pairs used in this study
| Function | Primer | Sequence (5′→3′) | Nucleotide positions | Product size (bp) | Reference |
|---|---|---|---|---|---|
| Screening of hdcA presence in Gram-positive bacteria | HIS1-F | GGNATNGTNWSNTAYGAYMGNGCNGA | 172–197a | 372 | 9 |
| HIS1-R | ATNGCDATNGCNSWCCANACNCCRTA | 520–545a | |||
| Screening of hdcA presence in S. thermophilus strains and RT-qPCR | STDEC-F | GAATTACCGATCTATGATGC | 349–368a | 121 | This study |
| STDEC-R | ACACCTTTGTTAGCACAAAC | 451–470a | |||
| RT-qPCR on the S. thermophilusrpoA | RPOST-F | ACTGTCATTGTTGCTTGGAATG | 521–542b | 114 | 13 |
| RPOST-R | AGCTGAGGTTACTGCTGGAGAT | 613–634b | |||
| Random primer for uneven PCR | Coc-1 | AGCAGCGTGG | This study | ||
| Random primer for uneven PCR | RDW | CCGATCTTCTCGC | This study |
a
Positions in the S. thermophilus PRI60 hdcA sequence.
b
Positions in the rpoA ORF of S. thermophilus LMD-9 (accession no. CP000419; region 1755506 to 1756444).