Table 2.
Samples prepared for the analysis of the labeled-leucine metabolic route in a semisynthetic medium
| Sample typea | Sugar in synthetic medium | Precursor(s) |
|---|---|---|
| SM Leu | Maltose | Leucine |
| SM Leu/Cit | Maltose | Citric acid and leucine |
| SM Leu/Glut | Maltose | Glutamic acid and leucine |
| SM Leu/3MBnala | Maltose | 3-Methylbutanal and leucine |
| SM Leu (pH 3.6) | Maltose (pH 3.6) | Leucine |
| SM Leu/Cit (pH 3.6) | Maltose (pH 3.6) | Citric acid and leucine |
| SM Leu/Glut (pH 3.6) | Maltose (pH 3.6) | Glutamic acid and leucine |
| SM Leu/3MBnal (pH 3.6) | Maltose (pH 3.6) | 3-Methylbutanal and leucine |
| SM Leu+ | Maltose | Leucine+ |
| SM Leu+/Cit | Maltose | Citric acid and leucine+ |
| SM Leu+/Glut | Maltose | Glutamic acid and leucine+ |
| SM Leu+/3MBnal | Maltose | 3-Methylbutanal and leucine+ |
| SM Leu+ (pH 3.6) | Maltose (pH 3.6) | Leucine+ |
| SM Leu+/Cit (pH 3.6) | Maltose (pH 3.6) | Citric acid and leucine+ |
| SM Leu+/Glut (pH 3.6) | Maltose (pH 3.6) | Glutamic acid and leucine+ |
| SM Leu+/3MBnal (pH 3.6) | Maltose (pH 3.6) | 3-Methylbutanal and leucine+ |
| SF Leu | Fructose | Leucine |
| SF Leu/Cit | Fructose | Citric acid and leucine |
| SF Leu/Glut | Fructose | Glutamic acid and leucine |
| SF Leu/3MBnal | Fructose | 3-Methylbutanal and leucine |
| SF Leu (pH 3.6) | Fructose (pH 3.6) | Leucine |
| SF Leu/Cit (pH 3.6) | Fructose (pH 3.6) | Citric acid and leucine |
| SF Leu/Glut (pH 3.6) | Fructose (pH 3.6) | Glutamic acid and leucine |
| SF Leu/3MBnal (pH 3.6) | Fructose (pH 3.6) | 3-Methylbutanal and leucine |
| SF Leu+ | Fructose | Leucine+ |
| SF Leu+/Cit | Fructose | Citric acid and leucine+ |
| SF Leu+/Glut | Fructose | Glutamic acid and leucine+ |
| SF Leu+/3MBnal | Fructose | 3-Methylbutanal and leucine+ |
| SF Leu+ (pH 3.6) | Fructose (pH 3.6) | Leucine+ |
| SF Leu+/Cit (pH 3.6) | Fructose (pH 3.6) | Citric acid and leucine+ |
| SF Leu+/Glut (pH 3.6) | Fructose (pH 3.6) | Glutamic acid and leucine+ |
| SF Leu+/3MBnal (pH 3.6) | Fructose (pH 3.6) | 3-Methylbutanal and leucine+ |
a
SM, synthetic medium with maltose; SF, synthetic medium with fructose; Leu, leucine; Leu+, l-leucine-5,5,5-d3; Cit, citrate; Glut, glutamate; 3MBnal, 3-methylbutanal.