Table 8.
Production of 2-methylbutanoic acid and 3-methylbutanoic acid by L. sanfranciscensis LSCE1a
| Sample conditionb | Production (mg/liter) of: |
|
|---|---|---|
| 2-Methylbutanoic acid (ion 74) | 3-Methylbutanoic acid (ion 60) | |
| SM Leu | NDb | 5.3 |
| SM Leu/Glut | ND | 3 |
| SM Leu/3MBnal | ND | 3.6 |
| SM Leu/Cit | ND | 3.3 |
| SF Leu | 2.0 | 3.1 |
| SF Leu/Glut | ND | 3.1 |
| SF Leu/3MBnal | ND | 4.1 |
| SF Leu/Cit | ND | 3.75 |
| SMH Leu | 2.5 | 3.8 |
| SMH Leu/Glut | ND | 4 |
| SMH Leu/3MBnal | ND | 4.2 |
| SMH Leu/Cit | ND | 3 |
| SFH Leu | 3 | 4.2 |
| SFH Leu/Glut | ND | 4 |
| SFH Leu/3MBnal | ND | 4.3 |
| SFH Leu/Cit | 3 | 3.5 |
a
Production of 2-methylbutanoic acid and 3-methylbutanoic acid was calculated based on the log areas of their characteristic ions 74 and 60, respectively. Shown are results from production in semisynthetic media with maltose (SM) and fructose (SF) at pH 5.8 and 3.6 (SMH and SFH, respectively). Leu, unlabeled leucine; Glut, glutamic acid; 3Mbnal, 3-methylbutanal; Cit, citrate. The data are means of three independent experiments. The coefficients of variability were lower than 4%.
b
ND, not detectable.