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. 2011 Apr;77(8):2582–2588. doi: 10.1128/AEM.01616-10

Fig. 2.

Fig. 2.

GC-MS chromatograms (single ion mode; m/z = 45) of extracts of a standard, GM medium, and samples taken at the end of the fermentation of wild-type and transformant cultures. Chromatogram 1, mixture of all three 2,3-BD stereoisomers; chromatogram 2, GM medium blank; chromatogram 3, fermentation of C. acetobutylicum ATCC 824; chromatogram 4, fermentation of C. acetobutylicum WUR; chromatogram 5, fermentation of C. acetobutylicum WUR harboring the empty vector (pMTL500E); chromatogram 6, fermentation of C. acetobutylicum WUR harboring the pWUR460 construct containing the acr gene. The retention time of the d-2,3-BD peak in chromatogram 6 is somewhat different due to the high concentration. Spiking experiments confirmed that it is indeed the d-stereoisomer.