Table 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant genotypea | Remark | Source |
|---|---|---|---|
| Bacterial strains | |||
| C. acetobutylicum WUR | WT | Originally obtained as ATCC 824, but shown to deviate from type strain behavior | Laboratory stock |
| C. acetobutylicum ATCC 824 | WT | Type strain | H. Bahl, Rostock, Germany |
| C. beijerinckii NCIMB 8052 | WT | Laboratory stock | |
| E. coli DH10B(pAN1) | Δ(mrr-hsdRMS-mcrBC) | Methylation strain | Laboratory stock |
| Plasmids | |||
| pAN1 | p15A ori; Cmr Φ3tI | Plasmid that expresses the phage Φ3tI methylase gene (18) | Laboratory stock |
| pMTL500E | ColE1 ori; pAMβ1 ori; MLSr Apr | Clostridial/E. coli shuttle vector (22) | Laboratory stock |
| pWUR459 | ColE1 ori; pAMβ1 ori; MLSr Apr; Padc-Cb-acr | Plasmid that expresses the Cb-acr gene under the control of the C. acetobutylicum acetoacetate decarboxylase promoter | This study |
| pWUR460 | ColE1 ori; pAMβ1 ori; MLSr Apr; Pthl-Cb-acr | Plasmid that expresses the Cb-acr gene under the control of the C. acetobutylicum thiolase promoter | This study |
a
Abbreviations: WT, wild type; ori, origin; Cmr, chloramphenicol resistance; MLSr, macrolide-lincosamide-streptogramin B resistance; Apr, ampicillin resistance; Cb-acr, C. beijerinckii acr (Cb-acr) gene.