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FACS DiOC2(3) red/green fluorescence histogram overlays showing the effect of selected compounds on the membrane potential of B. subtilis. Cells were exposed for 5 min to DMSO (CTRL), valinomycin, cereulide, CCCP, and the combination of valinomycin with nigericin. Treated cells are stained for 30 min with 30 μM DiOC2(3) probe.
