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Effect of storage on cultivability of aged C. difficile spores during 18 days of refrigeration at 4°C. (A) Results for nonheated spores illustrate reduced cultivability over time (12 strains; triangles and interval bars represent means and 95% CI). (B) Results for spores heated at 85°C indicate reproducible estimation and cultivability (PCR ribotype 078 strain; regression fits shown for 0, 3, 14, and 18 days).
