Table 3.
Gene dosage-dependent tightness and fold induction of the Tet-on system
| Run code | Strain used | Concn of Dox (μg/ml)a | LCPS/kgDWb before Dox addition (at 17 h) | Tightnessc (%) | LCPS/kgDW (at 26 h) | Fold inductiond |
|---|---|---|---|---|---|---|
| L0 + 5 | VG7.2 | 5 | 0.82 | 1.41 | ||
| L0 + 5 | VG7.2 | 5 | 0.80 | 1.35 | ||
| L1 + 0 | VG8.27 | 0 | 1.39 | 99.96 | 1.38 | 0 |
| L1 + 5 | VG8.27 | 5 | 1.52 | 99.96 | 1,316 | 950 |
| L1 + 5 | VG8.27 | 5 | 0.85 | 99.96 | 1,312 | 950 |
| L2 + 5 | VG8.1 | 5 | 9.21 | 99.82 | 4,466 | 3,200 |
| L3 + 5 | VG8.2 | 5 | 32.1 | 99.51 | 6,403 | 4,600 |
a
Dox was added after about 17.5 h run time.
b
DW, dry weight.
c
The tightness (%) of the system was calculated from the LCPS/kgDW values at 26 h (t2) and 17 h (t1) using the following formula: 100 − 100 × (LNt1 − L0t1)/(LNt2 − L0t2), where LN is L0, L1, L2, or L3.
d
Fold induction was calculated as follows: LNt2/(L1 + 0)t2.