Skip to main content
. 2001 Mar 1;15(5):535–553. doi: 10.1101/gad.855801

Figure 8.

Figure 8

The transforming activity of Rho is inhibited by dominant-negative c-Jun and kinases upstream of ERK6. (A) NIH-3T3 cells were transfected by the calcium phosphate technique with pCDNAIII β-Gal pCEFL–AU5-RhoA QL, or pCEV–MEK1 EE (1μg each), alone or in combination with c-Jun Tam67 (1 μg). Cells were cultured for 3 wk in 5% calf serum, and then fixed and stained. Representative plates for each transfection are shown. (B) Cells were transfected as above with with pCDNAIII β-Gal (1μg), pCEFL–AU5–RhoA QL (1 μg) or pCEFL–AU5 RasV12 (0.5 μg), alone or in combination with MKK3 AA, MKK6 KR, or MEK1 AA (1 μg each). The data represent percentage of foci developed with respect to those developed by RhoA QL or Ras V12 alone, whose values were taken as 100%. Results are the average ± s.e. of three experiments.