Table 2.

Oligonucleotide primers used for plasmid replicon typing

Name	Target site	Oligonucleotide primer sequence (5′–3′)		Amplicon size (bp)
		Forward	Reverse
HI1	parA-parB	GGAGCGATGGATTACTTCAGTAC	TGCCGTTTCACCTCGTGAGTA	471
HI2	Iterons	TTTCTCCTGAGTCACCTGTTAACAC	GGCTCACTACCGTTGTCATCCT	644
I1	RNAI	CGAAAGCCGGACGGCAGAA	TCGTCGTTCCGCCAAGTTCGT	139
X	oriγ	AACCTTAGAGGCTATTTAAGTTGCTGAT	TGAGAGTCAATTTTTATCTCATGTTTTAGC	376
L/M	RepA,B,C	GGATGAAAACTATCAGCATCTGAAG	CTGCAGGGGCGATTCTTTAGG	785
N	repA	GTCTAACGAGCTTACCGAAG	GTTTCAACTCTGCCAAGTTC	559
FIA	Iterons	CCATGCTGGTTCTAGAGAAGGTG	GTATATCCTTACTGGCTTCCGCAG	462
FIB	repA	GGAGTTCTGACACACGATTTTCTG	CTCCCGTCGCTTCAGGGCATT	702
W	repA	CCTAAGAACAACAAAGCCCCCG	GGTGCGCGGCATAGAACCGT	242
Y	repA	AATTCAAACAACACTGTGCAGCCTG	GCGAGAATGGACGATTACAAAACTTT	765
P	Iterons	CTATGGCCCTGCAAACGCGCCAGAAA	TCACGCGCCAGGGCGCAGCC	534
FIC	repA2	GTGAACTGGCAGATGAGGAAGG	TTCTCCTCGTCGCCAAACTAGAT	262
A/C	repA	GAGAACCAAAGACAAAGACCTGGA	ACGACAAACCTGAATTGCCTCCTT	465
T	repA	TTGGCCTGTTTGTGCCTAAACCAT	CGTTGATTACACTTAGCTTTGGAC	750
FIIS	repA	CTGTCGTAAGCTGATGGC	CTCTGCCACAAACTTCAGC	270
FrepB	RNAI/repA	TGATCGTTTAAGGAATTTTG	GAAGATCAGTCACACCATCC	270
K	RNAI	GCGGTCCGGAAAGCCAGAAAAC	TCTTTCACGAGCCCGCCAAA	160
B/O	RNAI	GCGGTCCGGAAAGCCAGAAAAC	TCTGCGTTCCGCCAAGTTCGA	159