Fig. 6.

Phylogenetic analysis of MCR amino acid sequences from the Napoli mud volcano sediments based on the neighbor-joining method with approximately 258 amino acid positions. The percent bootstrap values are based on 1,000 replicates and are indicated at the nodes for branch values with ≥50% bootstrap support. Gene sequences from the Napoli mud volcano sediments obtained in the present study are in boldface type. Clones with designations beginning with NapMat-2_4 are from the sediment section from 2 to 4 cmbsf, and clones with designations beginning with NapMat-8_10 are from the sediment section from 8 to 10 cmbsf. Numbers in parentheses indicate the number of analyzed clones that have >97% nucleotide sequence identity and >99% amino acid sequence identity. The scale bar indicates the 10% estimated difference. ANME, anaerobic methanotroph.