Table 2.
Plasmids used in the study
| Plasmid | Description/genotypea | Source or reference |
|---|---|---|
| pUC18 | Cloning vector (Ampr) | 41 |
| pET26b+ | Vector enabling IPTG-inducible expression of gene of interest with 6× C-terminal His tag (Kanr) | Novagen, Madison, WI |
| pDH32M | Integration vector for transcriptional fusions with lacZ at the amyE locus (Ampr Cmr) | 22 |
| pVD23 | pUC18 derivative containing homologous regions upstream of frlB and downstream of frlR flanking the Ermr gene (Ampr Ermr) | This work |
| pVD24 | pUC18 derivative containing homologous regions upstream and downstream of frlR flanking the Ermr gene (Ampr Ermr) | This work |
| pVD26 | pUC18 derivative containing homologous regions upstream and downstream of codY flanking the Kanr gene (Ampr Kanr) | This work |
| pVD28 | Fusion of pBC16-vector backbone and IPTG-inducible expression cassette of pHT01 (Mobitec) (Tcr) | This work |
| pVD30.1 | pET26b+ derivative containing codY gene (Kanr) | This work |
| pSKL3 | pET26b+ derivative containing frlR gene (Kanr) | This work |
| pVD33.1 | pVD28 derivative containing frlR gene | This work |
| pVD34 | pUC18 derivative containing homologous regions upstream and downstream of lacA flanking the Bler gene (Ampr Bler) | This work |
| pVD36 | pDH32M derivative containing the frlB promoter (Ampr Cmr) | This work |
| pVD37 | pDH32M derivative containing the frlR promoter (Ampr Cmr) | This work |
| pVD39 | pDH32M derivative containing the frlB promoter followed by the frlB gene and IfrlB-frlO (Ampr Cmr) | This work |
| pSKL5 | pDH32M derivative containing the hpaII promoter followed by the frlB gene and IfrlB-frlO (Ampr Cmr) | This work |
| pSKL6 | pDH32M derivative containing the hpaII promoter (Ampr Cmr) | This work |
a
IPTG, isopropyl-β-d-thiogalactopyranoside.