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Fluorescence microscopy of E. coli loaded for 1 h with 20 μM C12FDG in LB medium. (A) Cell suspension maintained at room temperature for 20 min. (B) Cell suspension heated to 40°C for 20 min. (C) A 1:1 mix of heated cells (as in panel B; examples indicated by red arrows) and room temperature cells (as in panel A; examples indicated by yellow arrows).
