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. 2001 Mar 1;15(5):581–590. doi: 10.1101/gad.867901

Figure 5.

Figure 5

Preference of the NTH15-binding sequence. (A) Core sequences of the NTH15-binding site found in fragments 1 and 7 (wt1 and wt2) and its derivative sequences used for the experiments (M1 to M8). Numbers at the top indicate the nucleotide positions. (B) electrophoresis mobility shift assay (EMSA) of each DNA fragment. Synthetic oligonucleotides containing the wild-type or mutagenized binding site were used as probes in EMSA. (C) NTH15 preferably interacted with the sequence containing G at position 6. The binding affinity was estimated with nonlabeled wt2 as a competitor (Comp.). The NTH15–DNA complexes are indicated by arrowheads.