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. 2001 Mar 1;15(5):581–590. doi: 10.1101/gad.867901

Figure 6.

Figure 6

NTH15-dependent repression of Ntc12 expression and the effect of two-base substitutions on Ntc12 repression. (A) Schematic structure of the promoter–luciferase (LUC) construct and nucleotide substitutions in the three mutants (Ntc12-m1 to Ntc12-m3) compared to the wild type (Ntc12-w.t.). (B) In vivo luminescence of LUC reporter was imaged using a high-sensitivity camera system. Ntc12-w.t. seedling was measured at 0 h, 3 h, 4 h, 5 h, and 6 h after dexamethasone (DEX) treatment. The appearance of treated seedling is shown in the left panel. (C) Ntc12-m1, Ntc12-m2, and Ntc12-m3 seedlings were measured 0 h and 6 h after DEX treatment. (D) Relative LUC activity 6 h after DEX treatment. The value obtained before DEX treatment was arbitrarily set at 100%. Each data point is the average of five plants.