Table 2.
Plasmids constructeda
| Plasmid | Description | Reference or source |
|---|---|---|
| p416-TEF | URA3, CEN6/ARSH4 origin, TEFp | 33 |
| p425-GPD | LEU2, 2μm origin, GPDp | 33 |
| p414-TEF | TRP, CEN6/ARSH4 origin, TEFp | 33 |
| p16T.X1 | p416-TEF-SsXyl1 | This study |
| p25G.X2 | p425-GPD-SsXyl2 | This study |
| p14T.02 | p414-TEF-AtXYLL3 | This study |
| p14T.03 | p414-TEF-AtXYLL2 | This study |
| p14T.04 | p414-TEF-ScHXT3 | This study |
| p14T.05 | p414-TEF-CiGXF1 | This study |
| p14T.06 | p414-TEF-CiGXS1 | This study |
| p14T.07 | p414-TEF-DEHA0D02167 | This study |
| p14T.08 | p414-TEF-DEHA2B14278 | This study |
| p14T.09 | p414-TEF-DEHA2A14300 | This study |
| p14T.10 | p414-TEF-DEHA2F19140 | This study |
| p14T.12 | p414-TEF-DhXylHP | This study |
| p14T.13 | p414-TEF-EcXylE | This study |
| p14T.14 | p414-TEF-SsXUT1 | This study |
| p14T.15 | p414-TEF-SsXUT2 | This study |
| p14T.16 | p414-TEF-SsXUT3 | This study |
| p14T.17 | p414-TEF-SsXUT4 | This study |
| p14T.18 | p414-TEF-SsXUT5 | This study |
| p14T.19 | p414-TEF-SsXUT6 | This study |
| p14T.20 | p414-TEF-SsXUT7 | This study |
| p14T.21 | p414-TEF-YALI0B06391 | This study |
| p14T.22 | p414-TEF-YALI0B01342 | This study |
| p14T.23 | p414-TEF-YALI0F06776 | This study |
| p14T.24 | p414-TEF-YALI0C06424 | This study |
| p14T.25 | p414-TEF-YALI0C08943 | This study |
| p14T.26 | p414-TEF-CNBC3990 | This study |
| p14T.27 | p414-TEF-ScHXT7 | This study |
| p14T.35 | p414-TEF-ScHXT13 | This study |
| p14T.36 | p414-TEF-ScGAL2 | This study |
a
The xylose metabolic genes XYL1 and XYL2 from Scheffersomyces stipitis were expressed in Mumberg et al. shuttle vectors (32), along with all known and putative transporter ORFs included in this study.