Table 2.
Fatty acid compositions of L. lactis subsp. cremoris MG1363 strains grown at 30°C on M17/7 medium or under sublethal stress conditions
| Fatty acidd | Fatty acid composition (molar %)a |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| M17/7 medium |
M17/5 medium |
M17/7 medium plus 6% ethanol |
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| Wild type | Δcfa strain | Δcfa + pDLcfa | Wild type | Δcfa strain | Δcfa + pDLcfa | Wild type | Δcfa strain | Δcfa + pDLcfa | |
| Iso-C15:0 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 6.84 ± 8.44 | 6.46 ± 3.19 | 13.33 ± 5.92 |
| C14:0 | 9.21 ± 0.35 | 9.91 ± 0.75 | 11.07 ± 1.45 | 7.46 ± 1.09 | 6.86 ± 0.97 | 8.82 ± 1.08 | 10.45 ± 2.13 | 8.92 ± 0.73 | 9.95 ± 1.21 |
| Anteiso-C15:0 | 5.15 ± 1.43 | 3.65 ± 0.28 | 3.77 ± 0.78 | 7.22 ± 0.50 | 5.71 ± 0.16 | 4.66 ± 3.58 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| C16:0 | 22.63 ± 2.08 | 24.04 ± 1.32 | 23.59 ± 1.92 | 29.53 ± 1.47 | 28.34 ± 0.95 | 33.22 ± 1.82 | 23.12 ± 3.27 | 26.20 ± 2.93 | 30.34 ± 1.70 |
| C16:1n-7 | 2.42 ± 0.53 | 2.16 ± 0.18 | 2.95 ± 1.31 | 2.13 ± 1.58 | 2.60 ± 1.84 | 0.00 ± 0.00 | 17.36 ± 5.76 | 16.45 ± 5.15 | 0.00 ± 0.00 |
| A4b | 7.59 ± 0.60 | 8.33 ± 0.46 | 3.96 ± 2.15 | 1.76 ± 1.44 | 16.23 ± 1.57 | 0.00 ± 0.00 | 0.40 ± 0.41 | 2.06 ± 0.81 | 0.00 ± 0.00 |
| C18:0 | 1.33 ± 0.51 | 1.63 ± 0.79 | 1.54 ± 0.40 | 3.44 ± 0.71 | 3.93 ± 0.64 | 5.11 ± 0.99 | 6.31 ± 1.62 | 8.14 ± 3.29 | 10.11 ± 0.58 |
| C18:1n-7 | 32.29 ± 5.67 | 50.28 ± 1.69 | 19.42 ± 4.86 | 3.60 ± 0.95 | 36.32 ± 1.91 | 0.00 ± 0.00 | 9.93 ± 1.72 | 31.77 ± 7.47 | 0.00 ± 0.00 |
| A5b | 1.23 ± 0.71 | 0.00 ± 0.00 | 4.70 ± 0.17 | 13.74 ± 0.67 | 0.00 ± 0.00 | 4.60 ± 2.86 | 0.27 ± 0.47 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| C19:0n-7 cyc | 18.15 ± 3.23 | 0.00 ± 0.00 | 29.00 ± 3.69 | 31.11 ± 0.43 | 0.00 ± 0.00 | 43.58 ± 2.89 | 25.32 ± 2.41 | 0.00 ± 0.00 | 36.28 ± 3.61 |
| CFA conversion (%)c | 35.99 | 0.00 | 59.89 | 89.62 | 0.00 | 100.00 | 71.83 | 0.00 | 100.00 |
a
Samples were taken from stationary phase of growth. Each value is the mean obtained from three separate analyses. The summed data presented is shown as the mean value ± standard deviation of three replicate values.
b
Unidentified fatty acids.
c
CFA conversion = 100 × [Σ(C19:0n-7 cyc)/Σ(C19:0n-7 cyc + C18:1n-7)].
d
C14:0, myristic acid; C16:0, palmitic acid; C16:1n-7, palmitoleic acid; C18:0, stearic acid; C18:1n-7, cis-vaccenic acid; C19:0n-7 cyc, lactobacillic acid.