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(A) Thermal inactivation of YdaE BL at 30°C (filled diamonds), 35°C (filled squares), 40°C (filled triangles), 45°C (circles), and 50°C (triangles). (B) Effects of metal ions on the activity of YdaE BL. The reaction was run in 50 mM Tris-Cl (pH 8.0) containing 10 mM d-mannose at 40°C. Data are means of three experiments.
