Table 1.
Phenotypic and genotypic characteristics of strains
| Strain | Source | Phylo- genetic group | Reference | fima | YAf | papa | papGUMN026a | papG83972a | papGIAI39a | HAb | Hemolysisc | Motility (mm)d | tcpCa | PAI-serU | sfa-foc |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MG1655 | Commensal | A | BEAICDFGH+ | + | − | − | − | − | − | − | 47 | − | − | − | |
| B2A | Urosepsis | B2 | This study | BEAICDFGH+ | + | IBAHCDJKEFG+ | + | − | − | + | + | 80 | + | + | C+ |
| B3 | Urosepsis | B2 | This study | BDFGH+ | − | IBAHCDJKEFG+ | + | + | − | − | + | 80 | − | − | CBADEFGH+ |
| B6 | Urosepsis | B2 | This study | BEAICDFGH+ | + | − | − | − | − | − | − | 76 | − | + | |
| B8 | Urosepsis | B2 | This study | BEAICDFGH+ | + | − | − | − | − | − | + | 80 | + | + | CBADEFGH+ |
| B18 | Urosepsis | B2 | This study | BEAICDFGH+ | + | IBCDJKEFG+ | − | + | − | − | + | 0 | + | + | C+ |
| B20 | Urosepsis | B2 | This study | BEACDFGH+ | + | IBAHCDJKEFG+ | + | − | − | + | − | 32 | − | − | |
| B26 | Urosepsis | B2 | This study | BEAICDFGH+ | + | IBAHCDJKEFG+ | + | − | − | + | − | 80 | + | + | CBADEFGH+ |
| B30 | Urosepsis | B2 | This study | BEAICDFGH+ | + | IBAHCDJKEFG+ | + | − | − | + | − | 77 | − | − | S+ |
| B31 | Urosepsis | B2 | This study | BEAICDFGH+ | + | IBHCDJKEFG+ | − | + | − | + | + | 0 | + | + | |
| B34 | Urosepsis | B2 | This study | BEAICDFGH+ | + | IBAHCDJKEFG+ | + | − | − | + | − | 31 | − | − | CBDEFSH+ |
| B37 | Urosepsis | B2 | This study | BEAICDFGH+ | + | IBAHCDJKEFG+ | + | + | − | − | + | 0 | + | + | CBADEFGH+ |
| B39 | Urosepsis | B2 | This study | BEAICDFGH+ | + | IBACDJKEFG+ | − | + | − | − | + | 0 | + | + | C+ |
| B40 | Urosepsis | B2 | This study | BEAICDFGH+ | + | IBCDJKEFG+ | − | + | − | − | + | 0 | + | + | CBADEFSH+ |
| B41 | Urosepsis | B2 | This study | BEACDFGH+ | + | IBAHCDJKEFG+ | + | − | − | + | − | 36 | − | − | |
| CFT073 | Urosepsis/pyelonephritis | B2 | 30 | BEAICDFGH+ | + | IBAHCDJKEFG+ | + | − | − | + | + | 19 | + | + | CBADEFGH+ |
| VR150 | Pyelonephritis | B2 | 14 | BEAICDFGH+ | + | F+ | − | − | − | − | + | 78 | + | + | CBADEFGH+ |
| VR151 | Pyelonephritis | B2 | 14 | BEAICDFGH+ | + | IBAHCDJKEFG+ | + | + | − | + | + | 10 | + | + | CBADEFGH+ |
| VR152 | Pyelonephritis | B1 | 14 | BEAICDFGH+ | + | A+ | − | − | − | − | − | 80 | − | − | |
| VR153 | Pyelonephritis | B2 | 14 | BEAICDFGH+ | + | IBAHCDJKEFG+ | + | − | − | + | − | 10 | − | − | |
| VR154 | Pyelonephritis | D | 14 | BEAICDFGH+ | + | IBAHCDJKEFG+ | + | − | − | + | − | 65 | − | − | |
| VR155 | Pyelonephritis | D | 14 | BEAICDFGH+ | + | IBAHCDJKEFG+ | + | − | − | + | − | 54 | − | − | |
| VR156 | Pyelonephritis | B2 | 14 | BEAICDFGH+ | + | IBAHCDJKEFG+ | + | + | − | + | + | 80 | − | − | CBADEFGH+ |
| VR157 | Pyelonephritis | B2 | 14 | BEACDFGH+ | + | IBCDJKEFG+ | + | − | − | + | + | 75 | + | + | C+ |
| VR145 | Cystitis | B2 | 40 | BEAICDFGH+ | + | − | − | − | − | − | + | 80 | − | − | CBADEFGH+ |
| VR146 | Cystitis | B2 | 40 | BEAICDFGH+ | + | I+ | − | − | − | − | + | 69 | − | − | CBDEFH+ |
| VR147 | Cystitis | D | This study | BEAICDFGH+ | + | IBAHCDJKEFG+ | + | − | − | − | − | 38 | − | − | |
| VR148 | Cystitis | A | This study | BEAICDFGH+ | − | − | − | − | − | − | − | 80 | − | − | |
| VR149 | Cystitis | D | This study | BEAICDFGH+ | + | − | − | − | − | − | − | 80 | − | − | |
| VR158 | Cystitis | B2 | This study | BEAICDFGH+ | + | − | − | − | − | − | − | 68 | − | + | |
| VR159 | Cystitis | B2 | 40 | BEAICDFGH+ | + | IBA+ | − | − | − | − | + | 0 | − | − | CBDEFH+ |
| VR160 | Cystitis | D | This study | BEAICDFGH+ | − | IBAHCDJKEFG+ | + | − | − | + | − | 58 | − | − | |
| VR161 | Cystitis | B1 | This study | BEAICDFGH+ | − | − | − | − | − | − | − | 80 | − | − | |
| 83972 | ABU | B2 | 1, 27 | BDFGH+ | − | IBAHCDJKEFG+ | − | + | − | − | − | 0 | − | − | CBADEFGH+ |
| VR11 | ABU | B2 | 7 | BEAICDFGH+ | + | − | − | − | − | − | + | 80 | − | − | CBADEFGH+ |
| VR12 | ABU | B1 | 7 | BEAICDFGH+ | + | − | − | − | − | − | − | 80 | − | − | |
| VR89 | ABU | D | 33 | BEAICDFGH+ | − | EFG++ | − | − | + | − | − | 0 | − | − | |
| VR90 | ABU | D | 33 | BEAICDFGH+ | + | IBAG++ | − | − | + | − | − | 0 | − | − | C+ |
| VR91 | ABU | B1 | 33 | DH+ | − | A+ | − | − | − | − | − | 70 | − | − | |
| VR92 | ABU | B1 | 33 | BEAICDFGH+ | + | − | − | − | − | − | − | 0 | − | − | |
| VR94 | ABU | B2 | 33 | BEAICDFGH+ | + | I+ | − | − | − | − | − | 0 | − | − | CBADEFSH+ |
| VR95 | ABU | A | 33 | BEAICDFGH+ | − | −e | − | − | − | − | − | 8 | − | − | |
| VR96 | ABU | B1 | 33 | H+ | − | − | − | − | − | − | − | 62 | − | − | |
| VR135 | ABU | B2 | 14 | BEAICDFGH+ | + | I+ | − | − | − | − | − | 57 | − | − | CBADEFSH+ |
| VR136 | ABU | B2 | 14 | BEACDFGH+ | + | − | − | − | − | − | + | 24 | + | + | CADEFGSH+ |
| VR137 | ABU | D | 14 | BEAICDFGH+ | + | IBAHCDJKFG+ | + | − | + | + | − | 0 | − | − | C+ |
a
Operon genes presented, based on CGH data. +, present; −, absent.
b
HA, hemagglutination. The same results were obtained with and without mannose. +, present; −, absent.
c
All nonhemolytic strains lacked the hlyABCD genes, according to CGH data, with the exception of strain 83972, which carries the genes but is unable to produce functional hemolysin due to sequence variations. +, present; −, absent.
d
Average motility on urine plates (four replicates); 80 indicates maximum motility (i.e., the plates were completely covered after 16 h).
e
Most likely carries some other variant of papG (33).
f
YA, yeast agglutination. +, present; −, absent.