Strains |
|
|
E. coli strains |
|
|
MG1655 |
F−rph-1
|
5 |
S17-1 λpir
|
recAthiprohsd(r− m+) RP4:2-Tc:Mu:Km Tn7 λpir Tpr Smr
|
16 |
S. plymuthica strains |
|
|
RVH1 |
Wild type; natural isolate |
20, 23
|
RVH1 splI
|
splI::aacC1 Gmr
|
22 |
RVH1 budB
|
budB::EZ-Tn5 Kmr
|
This study |
RVH1 budR
|
budR::cat Cmr
|
This study |
RVH1 budA::lacZ
|
RVH1 with chromosomal lacZ reporter fusion to budAB promoter; Kmr
|
This study |
RVH1 splI budA::lacZ
|
RVH1 splI with chromosomal lacZ reporter fusion to budAB promoter; Gmr Kmr
|
This study |
RVH1 budR budA::lacZ
|
RVH1 budR with chromosomal lacZ reporter fusion to budAB promoter; Cmr Kmr
|
This study |
Plasmids |
|
|
pTRC99a |
Cloning vector carrying IPTG-inducible trc promoter (Ptrc); Apr
|
2 |
pTRC99a-Ptrc-budAB
|
pTRC99a carrying the budAB operon fused downstream of Ptrc
|
This study |
pFPV25 |
Cloning vector carrying promoterless gfp; Apr
|
19 |
pFPV25-PbudR-gfp
|
pFPV25 carrying the budR promoter fused upstream of gfp
|
This study |
pUC18 |
Cloning vector; Apr
|
26 |
pUC18-budA::lacZ-kan
|
pUC18 carrying the budAB promoter fused to lacZ
|
This study |
pSF100 |
Pir-dependent replicon; Apr Kmr
|
13 |
pSF100-budR::cat
|
pSF100 carrying budR::cat
|
This study |
pSF100-budA::lacZ-kan
|
pSF100 carrying the lacZ reporter cloned into the budA locus |
This study |