Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant features | Reference |
|---|---|---|
| Strains | ||
| E. coli strains | ||
| MG1655 | F−rph-1 | 5 |
| S17-1 λpir | recAthiprohsd(r− m+) RP4:2-Tc:Mu:Km Tn7 λpir Tpr Smr | 16 |
| S. plymuthica strains | ||
| RVH1 | Wild type; natural isolate | 20, 23 |
| RVH1 splI | splI::aacC1 Gmr | 22 |
| RVH1 budB | budB::EZ-Tn5 Kmr | This study |
| RVH1 budR | budR::cat Cmr | This study |
| RVH1 budA::lacZ | RVH1 with chromosomal lacZ reporter fusion to budAB promoter; Kmr | This study |
| RVH1 splI budA::lacZ | RVH1 splI with chromosomal lacZ reporter fusion to budAB promoter; Gmr Kmr | This study |
| RVH1 budR budA::lacZ | RVH1 budR with chromosomal lacZ reporter fusion to budAB promoter; Cmr Kmr | This study |
| Plasmids | ||
| pTRC99a | Cloning vector carrying IPTG-inducible trc promoter (Ptrc); Apr | 2 |
| pTRC99a-Ptrc-budAB | pTRC99a carrying the budAB operon fused downstream of Ptrc | This study |
| pFPV25 | Cloning vector carrying promoterless gfp; Apr | 19 |
| pFPV25-PbudR-gfp | pFPV25 carrying the budR promoter fused upstream of gfp | This study |
| pUC18 | Cloning vector; Apr | 26 |
| pUC18-budA::lacZ-kan | pUC18 carrying the budAB promoter fused to lacZ | This study |
| pSF100 | Pir-dependent replicon; Apr Kmr | 13 |
| pSF100-budR::cat | pSF100 carrying budR::cat | This study |
| pSF100-budA::lacZ-kan | pSF100 carrying the lacZ reporter cloned into the budA locus | This study |