Table 2.
Primers used in this study
| Primer | Sequence |
|---|---|
| KAN-2 RP-1 | GCAATGTAACATCAGAGATTTTGAG |
| budR_FW | TCACTCCCAGCTCGGGGGCT |
| budB_Rev | TTAAATCATCTGGCTGAA |
| budA_Rev | CGTGGTCGAGCTGATAATCC |
| budA_FW | AACCGACCTTTGCCTTTCTC |
| budR_gap | CGCCCGCTATGTTGAAC |
| bud_intergen | GTTCAACATAGCGGGCG |
| budB_final | GATCTCCAACGGCCAGCAG |
| budA_Fus | CCCCTTAGAAACCCTGCACCAAATG |
| pUC47 | CGCCAGGGTTTTCCCAGTCACGAC |
| pUC24bis | GAGTCAGTGAGCGAGGAAGGC |
| budAB_start | ATGAACGAAAAGCAGGGTGG |
| budR_Fus | CCCCTTAGGCGTCATTCATCTTTGC |
| Up-bud-XhoI | CCCCCTCGAGCATTACGTACTCCTCCACCAG |
| Down-bud-XhoI | CCGGCTCGAGAAGCAGGGTTGTTCCTGTGCG |
| pKD3/4-P1-FW | GTGTAGGCTGGAGCTGCTTC |
| pKD3/4-P2-Rev | CATATGAATATCCTCCTTAG |
| lacZ-FW_XhoI | TCGGCTCGAGACCATGATTACGGATTCACT |
| lacZ-RV_XhoI | TCCGCTCGAGTTTTTGACACCAGACCAACT |
| Up-bud-SalI | GGGGGTCGACGGGGCCTACGGACGGTAAGAG |
| Down-bud-SalI | GCGCGTCGACCGCAGAACAGGTTCGGTGAAG |