Table 3.

Identities of 16S rRNA gene sequence-generated OTUs and the likely significant diet-associated T-RFLP-generated OTUs that they represent^b

T-RF (bp)	Taxonomic assignment	Assigned OTU(s)a	Dietary treatment	Gut section	Age(s) of birds (days)
144	Lachnospiraceae	140/142 and/or 144/146	Control	Cecum	5, 7, 17
150	Clostridiales	152	Antimicrobial	Ileum	12
154	Lactobacillus reuteri	152	Antimicrobial	Ileum	12
181	Lactobacillus crispatus	178/180	Antimicrobial	Ileum/cecum	3–17
189	Lactobacillus johnsonii	186/188	Control	Ileum	5–12, 17
198	Subdoligranulum sp.	198/200	Antimicrobial	Cecum	3–7
198	Subdoligranulum sp.	198/200	Antimicrobial	Cecum	3–7
222	Lachnospiraceae	220	Control	Ileum/cecum	3
286	Unclassified bacteria	284/286 and/or 286/288	Control	Ileum/cecum	5–17
288	Unclassified bacteria	284/286 and/or 286/288	Control	Ileum/cecum	5–17
289	Unclassified bacteria	284/286 and/or 286/288	Control	Ileum/cecum	5–17
289	Ruminococcaceae	284/286 and/or 286/288	Control	Ileum/cecum	5–17
298	Unclassified bacteria	296/298	Control	Cecum	5, 17
298	Clostridiales	296/298	Control	Cecum	5, 17
488	Oxalobacteraceae	480/482	Control	Cecum	5, 17
493	Unclassified bacteria	490/492	Antimicrobial	Ileum	3
				Cecum	17
494	Klebsiella sp.	490/492	Antimicrobial	Ileum	3
				Cecum	17
496	Enterobacteriaceae	490/492	Antimicrobial	Ileum	3
				Cecum	17

^aT-RFs were assigned to OTUs based on the closest nucleotide-length match or within ±6 bp.

^bAssociations of T-RFLP OTU with dietary treatment, gut section, and age are indicated.