Table 2.
Recovery of MS2 coliphage from NanoCeram filters using various eluting solutions
| Eluting solutiona | pH | No. of trials | Influent titer (log10/20 liters ± SD) | Eluate titer (log10/420 ml ± SD) | Elution efficiency (% ± SD) |
|---|---|---|---|---|---|
| A (3% beef extract) | 9.3 | 2 | 7.99 ± 0.17 | 7.49 ± 0.07 | 34 ± 18 |
| B (Gly) | 9.3 | 3 | 8.12 ± 1.57 | 5.37 ± 2.35 | 0.4 ± 0.5 |
| C (3% beef extract + Gly) | 9.3 | 2 | 7.57 ± 0.33 | 6.63 ± 0.31 | 12 ± 1 |
| D (PB + Gly) | 9.3 | 2 | 7.67 ± 0.07 | 7.07 ± 0.01 | 26 ± 5 |
| D (PB + Gly) | 7.5 | 3 | 7.93 ± 1.23 | 7.31 ± 1.13 | 24 ± 7 |
| E (PB + Gly + 0.3% Tween 80) | 9.3 | 2 | 7.48 ± 1.55 | 7.06 ± 1.58 | 37 ± 2 |
| F (1.0% NaPP + PB + Gly) | 9.3 | 5 | 8.14b ± 0.46 | 7.90 ± 0.45 | 57 ± 3 |
| F (1.0% NaPP + PB + Gly) | 7.5 | 2 | 7.83b ± 0.02 | 7.23 ± 0.09 | 26 ± 4 |
| F (1.0% NaPP + PB + Gly) | 9.3 | 3 | 4.58c ± 0.02 | 4.51 ± 0.06 | 86 ± 9 |
| G (0.1% NaPP + PB + Gly) | 9.3 | 3 | 7.82 ± 0.16 | 7.41 ± 0.21 | 40 ± 7 |
| H (0.6 M NaI + PB + Gly) | 9.3 | 2 | 8.17 ± 0.38 | 6.59 ± 0.67 | 3 ± 2 |
a
Gly, 0.05 M glycine; PB, phosphate buffer (3.8 mM Na2HPO4, 6.5 mM KH2PO4); NaPP, sodium polyphosphate.
b
High (∼108 PFU) influent titer.
c
Low (∼104 PFU) influent titer.