Fig. 1.

Sequence alignment of pESA3 and pCTU1, produced with the Artemis Comparison Tool (ACT). A schematic of each plasmid is shown above (or below) its corresponding ruler. The sequence of pESA3 was modified by rejoining the repA gene at the 3′ end, which is split in the GenBank sequence. The G+C content (as a percentage) is shown between the plasmid ORF schematic and the ACT homology output; pESA3 and pCTU1 have a mean G+C contents of 56.85% and 56.05%, respectively. Select genes or loci are shown in color as follows: eit (red), iuc (orange), parAB and repA (purple), integrase (black) and associated genes (white), cpa (teal), T6SS (blue), and FHA (brown). In the middle section, the red color indicates significant nucleotide homology, as determined by BlastN analysis, between pESA3 and pCTU1, and the location on each plasmid, for example eit, iuc, parAB, and repA. White indicates regions or loci present on one plasmid and absent on the other, e.g., cpa, T6SS, and FHA.