Fig. 4.
Effect of increasing numbers of input VZV-infected MeWo cells on infection of hESC-derived neurons. A total of 5 × 104 (A, D, and G), 2.5 × 105 (B, E, and H), or 106 (C, F, and I) VZVBAC-infected MeWo cells were seeded with the equivalent of 5 neurospheres in wells of a 24-well plate. Photomicrographs of living cultures were taken at 1 day (A to C), 4 days (D to F), or 7 days (G to I) after infection. One of the few fields containing cells at this time point is shown in panel I, as most the coverslip was devoid of cells. In this field, all individual neurons have peeled off, and the remaining clumps are virtually 100% infected with VZV. Bar, 200 μm.