Fig. 3.

Newly synthesized viral DNA genomes localized outside the BMRF1 core move to the inside. Pulse-chase labeling experiments were performed with Tet-BZLF1/B95-8 cells at 24 h after induction. Outlines of the experimental protocol are given at the tops of panels A and B. (A) Tet-BZLF1/B95-8 cells were pulse-labeled with CldU for 10 min at 24 h postinduction. (B) The pulse-labeled cells were washed, and then chased for 1 h (B). For the 2D images, cells were treated with 0.5% mCSK buffer and stained with anti-BMRF1 or -BALF2 (green) and anti-CldU (red) antibodies. These images show brightest-point projections of 60 images collected at 0.26-μm steps in the z axis. The same data are displayed as 3D topographical reconstructions of the BMRF1 or BALF2 protein and CldU-labeled viral DNA (left and middle panels, respectively). The right panels show 3D surface reconstruction images.