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. 2011 Jul;85(13):6127–6135. doi: 10.1128/JVI.00258-11

Fig. 4.

Fig. 4.

Homologous recombinational repair proteins are located both outside and inside BMRF1 cores. (A to C) Lytic replication-induced Tet-BZLF1/B95-8 cells were treated with mCSK buffer, fixed with 70% ethanol, and stained with combinations of the indicated antibodies as follows: (A) anti-BMRF1 (green) and anti-Mre11 (red) antibodies; (B) anti-BMRF1 (green) and anti-BRCA1 ser-1524 (red) antibodies; (C) anti-BMRF1 (green) and anti-Rad52 (red) antibodies. The 2D images show brightest-point projections of 60 images collected at 0.26-μm steps in the z axis. The same data are displayed as 3D topographical reconstructions of each protein (left and middle panels, respectively). The right panels shows 3D surface reconstruction images of both proteins indicated, showing the BMRF1 core covered by HRR proteins. (D) The cells were stained with anti-BMRF1 (green), anti-BALF2 (blue), and anti-phosphorylated RPA32 Ser-4/Ser-8 (red) antibodies. Top panels, 2D images showing a brightest-point projection of 60 images collected at 0.26-μm steps in the z axis. Middle panels, each combination of the merged image of the top panels. Bottom panels, each combination of merged 3D surface reconstruction images.