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Serum anti-C8166-CCR5 IgG in immunized animals that received HLA-containing vaccine (groups 1, 3, and 4). Anti-C8166-CCR5 IgG was detected by FITC-conjugated anti-human IgG secondary antibody, and the mean fluorescence intensity (MFI) was measured. (A) Anti-C8166-CCR5 at the time of challenge. (B) Correlation between anti-C8166-CCR5 IgG and neutralizing antibodies, in the presence of complement, in groups 1 and 3. The protected animals F42 and F44 are denoted with empty symbols.
