Fig. 6.

Induction of NDV- and APMV3-neutralizing serum antibodies by rAPMV3 vaccine constructs. Chickens were immunized once by the oculonasal route with the indicated rAPMV3 constructs given individually or in combination, and sera were taken 21 days later and analyzed for the ability to neutralize APMV-3 (a) or NDV strain LaSota (b). The serum neutralizing antibody titers were expressed as mean reciprocal log₂ titer (means ± standard errors of the mean [SEM]). Statistical differences were calculated by one-way ANOVA with P < 0.001. Alternatively, the sera collected from birds (lanes 1 to 10) showed NDV F- and HN protein-specific bands after Western blot analysis using purified NDV proteins as antigens. Lane 11 was used with preimmune serum (c).