Figure 2.

nkd autonomously regulates interommatidial bristle formation. (A) Wild-type eye margin at dorsum of head shows bristle suppression 0–1 ommatidial rows (blue brackets) from eye margin. (B) nkd^7E89 mutant eye shows suppression of bristles 3–5 ommatidial rows from eye margin. Occasional bristles are present closer to eye margin (yellow arrow). No bristle phenotype was seen with the weaker nkd^9G33 allele, whereas h¹ nkd^7H16 eyes are small and rough (not shown), possibly due to h/nkd interactions (Zeng et al. 2000) and hence could not be scored for this phenotype. (C,D) Nkd/GFP misexpression clones in sev-wg adult eyes. (C) Bristles are restored only in the region of the clone marked by GFP. (D) Cartoon depiction of nine adult eye clones (green color); vertical black lines represent bristles. (E) Cut nuclear localization in bristle cells of a wild-type pupal eye disc. (F,G) Restoration of bristle cells in a sev-wg pupal eye, revealed by Cut localization (red color), is confined to the region of the Nkd/GFP misexpression clone (green color); the clone begins at the edge of the disc (left) and continues inward (right). (H) Merged image of F and G shows that GFP and Cut colocalize in bristle cell precursor nuclei present in the clone.